#Clc genomics workbench 6.0 plus

NCBI/GenBank: The sequences obtained were submitted to GenBank NCBI under the following accession numbers: KT000353-KT000360 (HSVd), KP693444-KP693448 (GPGV), KT000346-KT000352 (GYSVd-1), KT000361-KT000362 (GFkV), KT000363-KT000365 (GLRaV-1), and KT000366-KT000371 (GRSPaV).įunding: This work was supported by COST FA1407, Ministry of Education, Youth and Sports, Ministry of Agriculture.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting Information files. Received: JAccepted: NovemPublished: December 13, 2016Ĭopyright: © 2016 Eichmeier et al. PLoS ONE 11(12):Įditor: Dragan Perovic, Julius Kühn-Institut, GERMANY Among the pathogens detected, the Grapevine rupestris vein feathering virus and Grapevine yellow speckle viroid 1 were recorded in the Czech Republic for the first time.Ĭitation: Eichmeier A, Komínková M, Komínek P, Baránek M (2016) Comprehensive Virus Detection Using Next Generation Sequencing in Grapevine Vascular Tissues of Plants Obtained from the Wine Regions of Bohemia and Moravia (Czech Republic). Using next generation sequencing of small RNA, the presence of 8 viruses and 2 viroids were detected in a set of eight grapevines therefore, confirming the high effectiveness of the technique in plant virology and producing results supporting previous data on multiple infected grapevines in Czech vineyards.

#Clc genomics workbench 6.0 plus

Eight previously partially-characterized grapevines of diverse varieties were selected and subjected to analysis: Chardonnay, Laurot, Guzal Kara, and rootstock Kober 125AA from the Moravia wine-producing region plus Müller-Thurgau and Pinot Noir from the Bohemia wine-producing region, both in the Czech Republic. The novel NGS approach was based on sequencing libraries of small RNA isolated from grapevine vascular tissues. Comprehensive next generation sequencing virus detection was used to detect the whole spectrum of viruses and viroids in selected grapevines from the Czech Republic.